P236 Siderophore production by the emerging cystic fibrosis pathogens of the genus Achromobacter

Achromobacter are opportunistic pathogens increasingly isolated in patients with cystic fibrosis (CF). Despite recent advances, their virulence factors remain incompletely studied. Particularly, ironchelating molecules called siderophores have not yet been studied CF isolates although being major bacterial factors. The aims of our study were: I) to characterize the production by spp. from patients, II) compare results strains non-CF search for specific characteristics that may be observed context. A total 162 were including 104 66 and 58 (blood, bronchoalveolar lavage fluid, others). species identified nrdA gene sequencing. Siderophores quantified liquid Chrome Azurol-Sulphonate assay. Results obtained different versus compared using Mann-Whitney test. Siderophore was 91.4% 94% 90% siderophore producers, producing significantly higher quantity than (sometimes as high a Pseudomonas aeruginosa strain). Among diversity identified, xylosoxidans is showing both highest proportion siderophore-producing produced. widely produced genus Achromobacter. Strains present could contribute overall these emerging pathogens. A. xylosoxidans, most frequently CF, displayed unique might its epidemiological success lung.